You can check for valve guide wear without removing the guides. It's called a "wobble test", to see how much the stem tip wobbles from side to side while the valve is in its seat (No springs).
Personally, I'd remove the mushrooming from the stem keeper slots, and reuse, if the guide wear is within limits. Unless this was a a Hi-Po build with non-stock cam and valve springs. Then it would be all new parts, assuming you want some sort of longevity from the engine.
Cheers,