The reason that DOT wants a bladder is for safety reasons, asphalt will chew through a fiberglass tank very fast, then you have a fire or explosion possible. Or the rider if still with the bike could get doused with gasoline. Lining the tank is purely for sealing fuel resistance protective layer for the fiberglass resin. It does very little to nothing for strength or fire control.
When making a mould, it will replicate every dimple and blemish of the original. The reason you build a female mould is so the exterior of the finished part is very smooth. It is easier to create a smooth finish for the exterior that way as your resin fills the weave and gives a nice finish. A lighter cloth for the first or second layer is used. This lessens what is called "imprint", that is where the weave of the cloth is visible in the molded surface. This is usually a problem on vacumn bagged parts where you are working with a male part you are fiberglassing.
You also layer your cloth with 45 degree bias from the previous layer for strength.
You wont get many parts out of a plaster mould and you will have to use a mould release to get the tank to release from the plaster. It would have to be a multisecned mould of at least five or six sections. 2 bottoms, left and right side plus a top for around the tank opening. If you are going to harvest a filler opening from a metal tank you will need to account for that with a flange in your mould. So, there are lots of things you need to decide and learn about. What temperatures will the bike see, as thermal rate of expansion is different between your metal part and the fiberglass. This could cause a seam failure.