You definitely do have to calibrate all gauges. You’re doing it right, connecting all gauges one at a time to the same cylinder.
I have the needle vacuum gauge ones, not the fluid in a tube ones. I feel better using them than when I used the blue fluid type. Those seemed to be more erratic. Maybe too sensitive?
Assuming all maintenance operations are completed correctly and the carbs have a decent bench sync and you have no air leaks, they should be fairly steady and syncing shouldn’t take more than 5 minutes.