Cylinder is on! Had a couple of brain farts on the way, but it's together now.
I checked the ring gaps and installed the rings, verifying that they all had the 'top' markings facing up, and the order was correct. I had my first very close OOPS when I realized just before sliding the cylinder down that #2 piston had the two upper rings swapped! Glad that didn't happen. So I fixed that up, installed the pistons in the cylinder, then lowered the cylinders (with pistons inside) onto the rods, sliding the middle two wrist pins and circlips in first, then the outer two. This is my first time assembling a motor this way, and it was actually very easy to do by myself. The big bore totally eliminate the chamfer at the bottom of the cylinder, so it'd be super easy to break a ring without a compressor. This made it easy to ensure the rings made it in safely.
Also, not too proud to admit the second major oops I had. I had the cylinder installed and was cleaning up when I realized I had all four pistons in 180 degrees backwards, with the intake side facing the exhaust!!

Thankfully it only took about 30-40 minutes to pull it apart, switch them around and get it all back together. I really like this method of top end rebuilding. Aligning the rod with the wrist pin is a little tricky, but not too bad.
Next up is cleaning up the gasket surface on the head, applying some Hondabond around the main oil supply and return seals, copper coat on the head gasket, and torque the head down. I've got new rubber dampers for the cam chain tensioner, some new camshaft sprocket bolts, and a few other goodies to install, plus a quick carb inspection before buttoning it all up and givin 'er some gas. I'm gone this weekend but hope to fire it up next week at my weekly moto shop night with the guys.